Detection of carbapenem resistant Escherichia coli in cattle and pigs

The aim of this study was to detect the presence carbapenem resistant Escherichia coli (E. coli) in cattle and pigs. Altogether, 100 samples were collected, 50 each from pigs including mastitic milk faecal healthy as well diarrhoeic slaughter specimens Bacteria isolated antibiotic sensitivity test carried out. Eight isolates E. obtained 15 phenotypic resistance patterns against carbapenems (ertapenem, imipenem meropenem) analysed. In cattle, four observed be sensitive all three whereas, carbapenems. intestinal showed From a total 10 pigs, four, one ertapenem, meropenem respectively. Among five intestine, isolate ertapenem no meropenem. Polymerase chain reaction (PCR) out find genes (blaSHV, blaCTX, blaTEM blaNDM) 23 results revealed that seven (58.3 per cent) blaSHV gene (33.3 contained blaCTX which encodes enzymes. Antibiogram using 14 commonly used antimicrobial agents varied multidrug among isolates.